BMC’s Technology Seminar Series returns Wednesday with Clontech. Clontech’s SMARTer system is what we use for doing RNAseq on samples with limited input. They have some new techniques coming out that may be of interest.
Technology Seminar Series – 2013/2014
Sponsored by the BioMicro Center
SMARTer® Solutions for Low Input Transcriptome Sequencing
Wednesday, October 23, 2013
Building 68-180, 12:00
Lunch will be provided
Next Generation Sequencing (NGS) has increased our understanding of biology by enabling highly sensitive RNA expression analysis throughout the transcriptome, across a wide dynamic range. Two powerful applications, single cell RNA-Seq and stranded RNA-Seq, have been the focus of considerable efforts in protocol innovation. By utilizing the template switching activity of reverse transcriptase, Clontech’s patented SMART™ technology has enabled researchers to analyze their most challenging samples. The original, dT-primed SMART-based RNA-Seq protocol, designed to work with high-quality RNA or whole cells, is the gold standard for single cell analysis. SMART’s applicability has recently been extended to noncoding RNA and mRNA from degraded samples, e.g., FFPE. Using modified SMART adapters together with random priming makes it possible to generate strand-specific sequencing libraries directly from RNA in under four hours. This eliminates the laborious enzymatic steps required by other stranded RNA-Seq methods, while maintaining the sensitivity and reproducibility characteristic of SMART. This presentation will outline these current SMART methods and detail their ability to provide unrivaled mappability, gene body coverage, strand specificity, and sensitivity.
The Technology Seminar Series was created to highlight technologies available at MIT though our shared facilities. Each month the Seminar Series will showcase a different technology in the facility and bring you up to date on the latest advances and future directions of the technology. We have asked the companies we have invited to bring their scientists to speak (not the sales team) so you have a chance to interact with them directly.